重组Anti-Bcl10抗体[ep605y] (ab40752)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [ep605y] to Bcl10
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Bcl10抗体[ep605y]
参阅全部 Bcl10 一抗 -
描述
兔单克隆抗体[ep605y] to Bcl10 -
宿主
Rabbit -
特异性
Not suitable for mouse IHC-P.
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经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human Bcl10 (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: Raji, WEHI-231, RAW 264.7 whole cell and mouse spleen tissue lysates. Flow Cyt (intra): Daudi cells. IP: Daudi and WEHI-231 whole cell lystates. IHC-P: Human colon carcinoma.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
ep605y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40752于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/30 - 1/50.
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说明 |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/30 - 1/50. |
靶标
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功能
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
组织特异性
Ubiquitous. -
疾病相关
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
序列相似性
Contains 1 CARD domain. -
翻译后修饰
Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
细胞定位
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 8915 Human
- Entrez Gene: 12042 Mouse
- Omim: 603517 Human
- SwissProt: O95999 Human
- SwissProt: Q9Z0H7 Mouse
- Unigene: 193516 Human
- Unigene: 239141 Mouse
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别名
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
图片
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32 kDa Bcl10 was immunoprecipitated from 0.35mg WEHI-231 (mouse B cell lymphoma B lymphocyte ), whole cell lysate with ab40752 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab40752 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1 (Input): WEHI-231 (mouse B cell lymphoma B lymphocyte ), whole cell lysate 10 μg.
Lane 2 (+): WEHI-231 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40752 in WEHI-231 whole cell lysate.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 102 seconds.
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32 kDa Bcl10 was immunoprecipitated from 0.35mg of Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate with ab40752 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab40752 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1 (Input): Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate 10 μg.
Lane 2 (+): Daudi whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40752 in Daudi whole cell lysate.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
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All lanes : Anti-Bcl10 antibody [ep605y] (ab40752) at 1/2000 dilution
Lane 1 : WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate
Lane 2 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse bone marrow lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Exposure time: 103 secondsBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
ab129002 was used as a loading control.
The expression profile observed is consistent with what has been described in the literature (PMID: 10319863).
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Anti-Bcl10 antibody [ep605y] (ab40752) at 1/2000 dilution + Raji cell lysate at 10 µg
Predicted band size: 31 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ab40752 at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling Bcl10 with purified ab40752 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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Purified ab40752 at 1/50 dilution (2µg) immunoprecipitating Bcl10 in Daudi whole cell lysate.
Lane 1 (input): Daudi (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab40752 + Daudi whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40752 in Daudi whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 32 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab40752 被引用在 1 文献中.
- Ginster S et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS One 12:e0169026 (2017). WB . PubMed: 28052131