Application
Western blot
Sample
Human Cell lysate - whole cell (U937 and PBMC)
Loading amount
4e+006 cells
Specification
U937 and PBMC
Gel Running Conditions
Reduced Denaturing (10% Gel)
Blocking step
2% Milk with 1% BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 2% milk with 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: AbD Serotec STAR54 Sheep anti-rabbit IgG:HRP
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: ~70 kDa
Positive control
Jurkat Whole Cell Lysate though no bands were seen in this lane
Additional data
Additional Notes
Lane 1 - Jurkat Whole Cell Lysate
Lane 2 - U937 Whole Cell Lysate
Lane 3 - PBMC sample 1 Whole Cell Lysate
Lane 4 - PBMC sample 2 Whole Cell Lysate
Lane 2 - U937 Whole Cell Lysate
Lane 3 - PBMC sample 1 Whole Cell Lysate
Lane 4 - PBMC sample 2 Whole Cell Lysate
Abcam response
This negative Abreview is currently being investigated by Abcam Technical Support. Please check here for an update response from Abcam.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 03 2008