Anti-Bag5抗体(ab97660)
Key features and details
- Rabbit polyclonal to Bag5
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Bag5抗体 -
描述
兔多克隆抗体to Bag5 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a sequence within amino acids 1-165 of Human Bag5 (NP_004864).
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阳性对照
- 293T, HeLa, MOLT4 and Raji cell lysates; A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97660于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. -
序列相似性
Contains 5 BAG domains. -
结构域
The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain. - Information by UniProt
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数据库链接
- Entrez Gene: 522854 Cow
- Entrez Gene: 9529 Human
- Entrez Gene: 70369 Mouse
- Entrez Gene: 366734 Rat
- Omim: 603885 Human
- SwissProt: Q2TA08 Cow
- SwissProt: Q9UL15 Human
- SwissProt: Q8CI32 Mouse
see all -
别名
- BAG family molecular chaperone regulator 5 antibody
- BAG-5 antibody
- BAG5 antibody
see all
图片
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All lanes : Anti-Bag5 antibody (ab97660) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa
10% SDS-PAGE. -
ab97660 at 1/200 dilution staining Bag5 in A549 cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab97660 被引用在 5 文献中.
- Wang M et al. miRNA-770-5p expression is upregulated in patients with type 2 diabetes and miRNA-770-5p knockdown protects pancreatic ß-cell function via targeting BAG5 expression. Exp Ther Med 22:664 (2021). PubMed: 33986829
- Fan X et al. MiRNA-429 alleviates ketamine-induced neurotoxicity through targeting BAG5. Environ Toxicol 36:620-627 (2021). PubMed: 33283947
- Lin H et al. miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A. Oxid Med Cell Longev 2021:9230435 (2021). PubMed: 34777698
- Xi J et al. miR-155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling. Mol Med Rep 21:1011-1020 (2020). PubMed: 31922242
- Bruchmann A et al. Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis. BMC Cancer 13:96 (2013). WB, ICC/IF ; Human . PubMed: 23448667