Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Sample
Cow Cell lysate - whole cell (Normal mammary epithelial cells (nMEC))
Specification
Normal mammary epithelial cells (nMEC)
Treatment
1.0 uM ANS +/- 100 ng/mL IGF-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in 5% milk in TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-Rabbit IgG, Horseradish Peroxidase Linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Dilution
1/2500
Detection
Detection method
ECL
Bands
Specific: 18 kDa
Exposure
30 second(s)
Additional data
Additional Notes
Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 27 2013