Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Fibroblasts transduced with BSD-resistance gene)
Specification
Fibroblasts transduced with BSD-resistance gene
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citrate (pH 6.)
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Biotinylated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/500
Additional data
Abcam response
Thanks for testing ab38307 in IHC-P and for your honest feedback. As this application has not been previously tested, it may be that ab38307 is not useful for staining paraffin embedded/formalin fixed tissues. Or perhaps further optimization of staining conditions is required.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 08 2012