Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (bone marrow derived macrophages)
Specification
bone marrow derived macrophages
Negative control
rabbit IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
PU.1 antibody
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
1 ug antibody, 70 millions cells
sequencing of the IP DNA is ongoing
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Silvia Bonifacio
Verified customer
提交于 May 02 2014