Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Human Cell (Human colon cancer cell line)
Specification
Human colon cancer cell line
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.1% Triton-X 100 + 1% BSA
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat Anti-mouse IgG (H+L) (Molecul
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
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提交于 Oct 15 2013