Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa and SK-N-SH)
Specification
HeLa and SK-N-SH
Fixative
Paraformaldehyde
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
This antibody does not give the nuclear repair foci that are expected in both HeLa and SK-N-SH cells following bleomycin treatment and therefore does not appear to recognize BRCA1 in these cell lines by this IF methodology.
Abcam response
This antibody does not seem suitable to use in ICC/IF. However, please note that Abcam would also recommend using ab17251 at a higher concentrations (1/15 -1/50) than the ones used in this experiment.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Nov 30 2006