重组Anti-BOB1抗体[EPR3299] (ab92315)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3299] to BOB1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BOB1抗体[EPR3299]
参阅全部 BOB1 一抗 -
描述
兔单克隆抗体[EPR3299] to BOB1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human BOB1 aa 50-150. The exact sequence is proprietary.
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阳性对照
- Ramos, Daudi, NAMALWA or Raji nuclear lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3299 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92315于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 28 kDa. |
靶标
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功能
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. -
组织特异性
B-cell specific. -
疾病相关
Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. -
序列相似性
Belongs to the POU2AF1 family. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5450 Human
- Omim: 601206 Human
- SwissProt: Q16633 Human
- Unigene: 654525 Human
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别名
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
图片
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All lanes : Anti-BOB1 antibody [EPR3299] (ab92315) at 1/2000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : NAMALWA cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab92315 被引用在 1 文献中.
- Chu CS et al. Unique Immune Cell Coactivators Specify Locus Control Region Function and Cell Stage. Mol Cell 80:845-861.e10 (2020). PubMed: 33232656