Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblasts)
Specification
Fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/400
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 1% BSA in PBS + 0.1% Triton
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti Mouse Alexafluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/1000
Additional data
Additional Notes
Fixation in 2% paraformaldehyde for 30 mins at RT
Treated cells with 50 mM NH4Cl in PBS for 10 minutes to quench free aldehyde groups after permeabilisation.
Treated cells with 50 mM NH4Cl in PBS for 10 minutes to quench free aldehyde groups after permeabilisation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sharon Sneddon
Verified customer
提交于 Nov 30 2011