Application
Western blot
Sample
Rat Tissue lysate - whole (Adult Testes, brain)
Loading amount
20 µg
Specification
Adult Testes, brain
Gel Running Conditions
Reduced Denaturing (10% Poly acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1 % milk in 1x TBST
Secondary antibody
Name
Non-Abcam antibody was used: DAKO Anti rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~46 kDa kDa
Positive control
Testes. BMP8a expressed in Testes but no band observed in Testes but clean band in Brain lysate.
Lane 1 - Marker
Lane 2 - Adult Rat Testes
Lane 3 - Adult Rat Testes
Lane 4 - Adult Rat Brain
Lane 1 - Marker
Lane 2 - Adult Rat Testes
Lane 3 - Adult Rat Testes
Lane 4 - Adult Rat Brain
Additional data
Additional Notes
Very good clean antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 Aug 03 2012