Anti-BLNK抗体(ab2230)
Key features and details
- Goat polyclonal to BLNK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BLNK抗体
参阅全部 BLNK 一抗 -
描述
山羊多克隆抗体to BLNK -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide: KDSTRLKYAVKVS, corresponding to C terminal amino acids 444-456 of Human BLNK.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.
A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate. |
说明 |
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WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa. A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate. |
靶标
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功能
Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis. -
组织特异性
Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. -
疾病相关
Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
序列相似性
Contains 1 SH2 domain. -
翻译后修饰
Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways. -
细胞定位
Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction. - Information by UniProt
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数据库链接
- Entrez Gene: 29760 Human
- Entrez Gene: 17060 Mouse
- Omim: 604515 Human
- SwissProt: Q8WV28 Human
- SwissProt: Q9QUN3 Mouse
- Unigene: 665244 Human
- Unigene: 9749 Mouse
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别名
- AGM4 antibody
- B cell adapter containing SH2 domain protein antibody
- B cell adapter containing Src homology 2 domain protein antibody
see all
图片
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Anti-BLNK antibody (ab2230) at 4 µg/ml + Cell lysate prepared from human Daudi cells at 35 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Ab2230 staining (4µ g/ml) of Daudi lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Primary incubated for 1 hour. -
All lanes : Anti-BLNK antibody (ab2230) at 1 µg/ml
Lane 1 : Cell lysate prepared from HEK293 mock transfected cells
Lane 2 : Cell lysate prepared from HEK293 overexpressing BLNK
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (0)
ab2230 尚未被引用在任何文献中。