Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Rhesus monkey Tissue sections (Colon)
Specification
Colon
Permeabilization
Yes - 0.1X Triton-X-100
Fixative
Z-fix
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10mM Tris-HCl and 150mM NaCl
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa 488 flurophor
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
The signal was good not intense in the colonic epithelium at 1:200. We think reducing the dilution to 1:100 will strengthen the signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 11 2014