Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (gel 12%)
Sample
Human Cell lysate - whole cell (muscle)
Specification
muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-BSA 2%
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-rabbit IgG horseradish peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
SuperSignal West Femto Chemiluminescent
Exposure
2 minute(s) and 0 second(s)
Positive control
No
Additional data
Abcam response
We are sorry that this antibody did not provide satisfactory results for this customer.
Customer received a free antibody in compensation as per our Abpromise guarantee.
We would like to encourage other customers to send us their feedback about the use of this antibody in WB and human. Please contact our Scientific Support team if you have any questions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 30 2014