Anti-BCKDK抗体(ab111716)
Key features and details
- Rabbit polyclonal to BCKDK
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-BCKDK抗体
参阅全部 BCKDK 一抗 -
描述
兔多克隆抗体to BCKDK -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human BCKDK aa 46-287. (AAH07363)
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阳性对照
- 293T and Mouse brain whole cell lysates; Human gastric carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab111716 is purified by antigen-affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111716于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0) |
靶标
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功能
Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 10295 Human
- Entrez Gene: 12041 Mouse
- Omim: 614901 Human
- SwissProt: O14874 Human
- SwissProt: O55028 Mouse
- Unigene: 513520 Human
- Unigene: 425495 Mouse
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别名
- [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody
- [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody
- BCKD kinase antibody
see all
图片
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Anti-BCKDK antibody (ab111716) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS-PAGE. -
Anti-BCKDK antibody (ab111716) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 46 kDa
10% SDS-PAGE. -
ab111716 at 1/500 dilution staining BCKDK in Formalin-fixed, paraffin-embedded Human Gastric carcinoma tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab111716 被引用在 2 文献中.
- Bravo-Alonso I et al. Dataset reporting BCKDK interference in a BCAA-catabolism restricted environment. Data Brief 7:755-9 (2016). WB . PubMed: 27054191
- García-Cazorla A et al. Two novel mutations in the BCKDK (branched-chain keto-acid dehydrogenase kinase) gene are responsible for a neurobehavioral deficit in two pediatric unrelated patients. Hum Mutat 35:470-7 (2014). PubMed: 24449431