BCA蛋白Assay 试剂盒(ab102536)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Assay time: 1 hr 30 min
- Sample type: Cell culture extracts, Plasma, Serum, Tissue Extracts
- Sensitivity: 0.5 µg
概述
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产品名称
BCA蛋白Assay 试剂盒
参阅全部 BCA Protein 试剂盒 -
检测方法
Colorimetric -
样品类型
Serum, Plasma, Cell culture extracts, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 0.5 µg -
范围
0.5 µg - 30 µg -
检测时间
1h 30m -
产品概述
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).
The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.
The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 µg protein (0.01-0.6 mg/ml).
Support on how to determine protein concentration can be found here.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1000 tests BCA Reagent 1 x 100ml BSA Standard III 1 x 1ml Copper Reagent 1 x 2ml -
研究领域
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相关性
The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration. -
别名
- BCA
- Bicinchoninic acid
- Bicinchoninic acid protein
数据表及文件
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SDS download
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Datasheet download
文献 (136)
ab102536 被引用在 136 文献中.
- Xu J et al. Carvedilol alleviates lipopolysaccharide (LPS)-induced acute lung injury by inhibiting Ras homolog family member A (RhoA)/ROCK activities. Bioengineered 13:4137-4145 (2022). PubMed: 35188451
- Liu M et al. miR-197 Participates in Lipopolysaccharide-Induced Cardiomyocyte Injury by Modulating SIRT1. Cardiol Res Pract 2022:7687154 (2022). PubMed: 35223094
- Yao D et al. circHIPK3 regulates cell proliferation and migration by sponging microRNA-124 and regulating serine/threonine kinase 3 expression in esophageal squamous cell carcinoma. Bioengineered 13:9767-9780 (2022). PubMed: 35443871
- Chen X et al. Circ_0014359 promotes oral squamous cell carcinoma progression by sponging miR-149. Acta Biochim Pol 69:315-320 (2022). PubMed: 35612554
- Chi A et al. ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory Stress. Front Endocrinol (Lausanne) 13:871548 (2022). PubMed: 35634492