Anti-BARD1抗体[2059C4a] (ab50984)
Key features and details
- Mouse monoclonal [2059C4a] to BARD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-BARD1抗体[2059C4a]
参阅全部 BARD1 一抗 -
描述
小鼠单克隆抗体[2059C4a] to BARD1 -
宿主
Mouse -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (Human)
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阳性对照
- HEK293 whole cell lysate, RIPA lysate of HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Protein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22 µm membrane. -
克隆
单克隆 -
克隆编号
2059C4a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/50. Predicted molecular weight: 86 kDa.
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IP |
1/50.
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说明 |
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WB
1/50. Predicted molecular weight: 86 kDa. |
IP
1/50. |
靶标
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功能
Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 4 ANK repeats.
Contains 2 BRCT domains.
Contains 1 RING-type zinc finger. -
翻译后修饰
Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer. -
细胞定位
Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex. - Information by UniProt
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数据库链接
- Entrez Gene: 580 Human
- Omim: 601593 Human
- SwissProt: Q99728 Human
- Unigene: 591642 Human
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别名
- BARD-1 antibody
- Bard1 antibody
- BARD1_HUMAN antibody
see all
图片
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Anti-BARD1 antibody [2059C4a] (ab50984) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Ab50984 at 1/50 dilution precipitating RIPA lysate of HeLa cells at 500µg; visualised with anti mouse IgG antibody at 1/2500 diltuion.
数据表及文件
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Datasheet download
文献 (3)
ab50984 被引用在 3 文献中.
- Gan C et al. USP25 inhibits DNA damage by stabilizing BARD1 protein in a house dust mite-induced asthmatic model in vitro and in vivo. Cell Biol Int 46:922-932 (2022). PubMed: 35143098
- Maurer LM et al. BRCA1-associated RING domain-1 (BARD1) loss and GBP1 expression enhance sensitivity to DNA damage in Ewing sarcoma. Cancer Res Commun 2:220-232 (2022). PubMed: 36187937
- Stecklein SR et al. BRCA1 and HSP90 cooperate in homologous and non-homologous DNA double-strand-break repair and G2/M checkpoint activation. Proc Natl Acad Sci U S A 109:13650-5 (2012). WB . PubMed: 22869732