Application
Flow Cytometry
Sample
Human Cell (HeLa)
Specification
HeLa
Preparation
Cell harvesting/tissue preparation method: Cells were collected by trypsinization
Sample buffer: PBS
Sample buffer: PBS
Fixation
no fixation
Permeabilization
No
Other product details
Dilution
1/100
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Abcam antibody:Donkey anti-Mouse IgG H&L (DyLight® 488) secondary antibody
Dilution
1/200
Additional data
Additional Notes
HeLa cells, treated with siRNA against Axl, were trypsinized and stained with anti-Axl 1:100 for 45 min on ice. cells treated with control siRNA show a high level of Axl.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Nir Drayman
Verified customer
提交于 Nov 23 2011