Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse E10.5 embryo)
Specification
mouse E10.5 embryo
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step
NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 5% donkey serum + PBST
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555 donkey anti rabbit (Invitrogen)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/1000
Additional data
Additional Notes
The image is of a transverse section through an E10.5 embryo, showing the neural tube. According to published ISH data, there is Axin-2 mRNA expression in dorsal neural tube and somites, but not all over the embryo.
The antibody has only been tested in embryo sections and the unspecific staining may be a result of how we process the sections.
We notice that staining embryo sections is more difficult than staining cells. Some antibodies work well in cells but not sections. However, we have not tested this antibody on cells.
The antibody has only been tested in embryo sections and the unspecific staining may be a result of how we process the sections.
We notice that staining embryo sections is more difficult than staining cells. Some antibodies work well in cells but not sections. However, we have not tested this antibody on cells.
Abcam response
Thank you very much for sharing this data with us. Abcam have not tested this antibody in IHC-Fr on mouse tissue. However, previous customers have successfully used this product both on mouse tissue and in IHC-Fr (please see abreviews). We are concerned there maybe some batch to batch variation in this product and would welcome feedback from other customers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 08 2012