Application
Western blot
Loading amount
3.75e+006 cells
Gel Running Conditions
Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
Specification
whole cell extract
Blocking step
0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: under slow agitation
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Goat polyclonal to rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Bands
Specific: 87 kDa Non-specific: 60, 30, 20 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
whole cell extract of S. cerevisiae
Additional data
Abcam response
Thanks for testing ab12099 for reactivity to S pombe. Based on this data it appears that ab12099 does not cross react with S pombe Arp5.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 17 2013