Anti-Arp5抗体(ab12099)
Key features and details
- Rabbit polyclonal to Arp5
- Suitable for: WB
- Knockout validated
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
概述
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产品名称
Anti-Arp5抗体 -
描述
兔多克隆抗体to Arp5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Recombinant full length protein corresponding to Arp5.
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阳性对照
- S. cereviseae lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12099于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
靶标
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相关性
The budding yeast INO80 complex is a conserved ATP-dependent nucleosome remodeler containing actin-related proteins Arp5 and Arp8. Strains lacking INO80, ARP5, or ARP8 have defects in transcription. Ino80, Arp5, and Arp8 are recruited to an HO-induced DSB, where a phosphorylated form of H2A accumulates. Recruitment of Ino80 is compromised in cells lacking the H2A phosphoacceptor S129. INO80-mediated chromatin remodeling seems to occur directly at DSBs, where it appears to facilitate processing of the lesion. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 855665 Saccharomyces cerevisiae
- SwissProt: P53946 Saccharomyces cerevisiae
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别名
- Actin-like protein ARP5 antibody
- ARP5_YEAST antibody
- P53946 antibody
图片
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Anti-Arp5 antibody (ab12099) + S.cerevisiae lysate
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 87 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa (possible cross reactivity) -
All lanes : Anti-Arp5 antibody (ab12099) at 0.36 µg/ml
Lane 1 : S. cerevisiae whole cell lysate (wild type)
Lane 2 : S. cerevisiae whole cell lysate (arp5 knockout)
Lane 3 : S. cerevisiae whole cell lysate (arp8 knockout)
Lane 4 : S. cerevisiae whole cell lysate (ino80 knockout)
Lane 5 : S. cerevisiae whole cell lysate (anc1 knockout)
Lane 6 : S. cerevisiae whole cell lysate (nhp10 knockout)
Lane 7 : S. cerevisiae whole cell lysate (wild type, treated with MMS)
Lane 8 : S. cerevisiae whole cell lysate (arp8 knockout, treated with MMS)
Predicted band size: 87 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Using ab12099, bands were present in all S. cerevisiae strains tested except for an arp5 knockout strain. This provides conclusive evidence that the ab12099 recognises Arp5.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab12099 被引用在 9 文献中.
- Gowans GJ et al. INO80 Chromatin Remodeling Coordinates Metabolic Homeostasis with Cell Division. Cell Rep 22:611-623 (2018). WB ; Saccharomyces cerevisiae . PubMed: 29346761
- Xue Y et al. Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals. Mol Cell 67:594-607.e4 (2017). PubMed: 28735899
- Panday A & Grove A The high mobility group protein HMO1 functions as a linker histone in yeast. Epigenetics Chromatin 9:13 (2016). ChIP . PubMed: 27030801
- Billant O et al. The dominant-negative interplay between p53, p63 and p73: A family affair. Oncotarget 7:69549-69564 (2016). WB . PubMed: 27589690
- Bennett G & Peterson CL SWI/SNF recruitment to a DNA double-strand break by the NuA4 and Gcn5 histone acetyltransferases. DNA Repair (Amst) 30:38-45 (2015). PubMed: 25869823
- Panday A et al. Yeast high mobility group protein HMO1 stabilizes chromatin and is evicted during repair of DNA double strand breaks. Nucleic Acids Res 43:5759-70 (2015). ChIP . PubMed: 25979266
- Xue Y et al. The Ino80 complex prevents invasion of euchromatin into silent chromatin. Genes Dev 29:350-5 (2015). ChIP-sequencing . PubMed: 25691465
- Yao W et al. Assembly of the Arp5 (Actin-related Protein) Subunit Involved in Distinct INO80 Chromatin Remodeling Activities. J Biol Chem 290:25700-9 (2015). PubMed: 26306040
- Bennett G et al. DNA repair choice defines a common pathway for recruitment of chromatin regulators. Nat Commun 4:2084 (2013). PubMed: 23811932