Application
Western blot
Sample
Rat Tissue lysate - whole (Testis)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
Testis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% NFDM
Dilution
1/1000
Secondary antibody
Dilution
1/40000
Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Rat testicular lysate without primary antibody (Anti Arp2) and with secondary antibody (Goat anti rabbit)
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Additional data
Additional Notes
85962
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Anita Kumar
Verified customer
提交于 Aug 12 2015