Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (HEK cells transfected with AQP5-EGFP)
Permeabilization
Yes - 0,2% Saponin
Specification
HEK cells transfected with AQP5-EGFP
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/200
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) 2nd AB Life Technolog
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
HEK cells were transfected with AQP5 (cDNA from rat lung) C-terminal tagged with EGFP
ab78486 was used at a concentration 1/200 and labeled with Alexa Fluor 568 (1/400)
Abcam response
Thank you for participating in our Abtrial program and you valuable feedback. We are sorry that this antibody did not work well for you in ICC, an application that has not been tested before. We welcome any further feedback and Abreviews from other customers who have used this antibody in ICC which will help us assess if the antibody works in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 03 2015