Anti-Aquaporin 1抗体(ab65837)
Key features and details
- Rabbit polyclonal to Aquaporin 1
- Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt (Intra)
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Aquaporin 1抗体
参阅全部 Aquaporin 1 一抗 -
描述
兔多克隆抗体to Aquaporin 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IHC-Fr, Flow Cyt (Intra)more details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Aquaporin 1 (C terminal).
Database link: P29972 -
阳性对照
- WB: Rat Kidney and lung tissue lysate, SMMC cell Lysate. Flow Cyt (Intra): U20S cells. IHC-P: Rat Kidney tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2% Trehalose -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65837于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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Flow Cyt (Intra) |
Use 1-3µg for 106 cells.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 29 kDa). |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. |
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
Flow Cyt (Intra)
Use 1-3µg for 106 cells. |
靶标
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功能
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
组织特异性
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
序列相似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
结构域
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 358 Human
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: P29975 Rat
- Unigene: 76152 Human
- Unigene: 1618 Rat
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别名
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
图片
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Aquaporin 1 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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All lanes : Anti-Aquaporin 1 antibody (ab65837) at 0.5 µg/ml
Lane 1 : Rat Kidney Tissue Lysate with 5% Non-fat Milk/ TBS
Lane 2 : Rat LungTissue Lysate with 5% Non-fat Milk/ TBS
Lane 3 : SMMC Cell Lysate with 5% Non-fat Milk/ TBS
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 29 kDa -
Overlay histogram showing U20S cells stained with ab65837 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 1 Antibody (ab65837,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Aquaporin 1 was detected in frozen section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Aquaporin 1 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
数据表及文件
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Datasheet download
文献 (11)
ab65837 被引用在 11 文献中.
- Ying Y et al. Activation of ATF4 triggers trabecular meshwork cell dysfunction and apoptosis in POAG. Aging (Albany NY) 13:8628-8642 (2021). PubMed: 33714955
- Sheng Z et al. Zika virus disrupts the barrier structure and Absorption/Secretion functions of the epididymis in mice. PLoS Negl Trop Dis 15:e0009211 (2021). PubMed: 33667230
- Manning JA et al. The ubiquitin ligase NEDD4-2/NEDD4L regulates both sodium homeostasis and fibrotic signaling to prevent end-stage renal disease. Cell Death Dis 12:398 (2021). PubMed: 33854040
- Fan JR et al. Lamin A-mediated nuclear lamina integrity is required for proper ciliogenesis. EMBO Rep 21:e49680 (2020). PubMed: 32815283
- Shu C et al. Inhibitory effect of AQP1 silencing on adhesion and angiogenesis in ectopic endometrial cells of mice with endometriosis through activating the Wnt signaling pathway. Cell Cycle 18:2026-2039 (2019). PubMed: 31251110