Application
Western blot
Sample
Human Cell lysate - whole cell (Mono-Mac 6)
Loading amount
50 µg
Specification
Mono-Mac 6
Treatment
TO-901317
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit HRP-linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: ~36 kDa Non-specific: ~45 kDa
Additional data
Additional Notes
Secondary antibody in 2.5% BSA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Elizabeth Kilby
Verified customer
提交于 Dec 18 2012