Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary astrocytes culture)
Permeabilization
Yes - 0.1M PBS with 1% Triton X
Specification
Primary astrocytes culture
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Methanol
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Alexa 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Rat primary Astrocyte culture stained with anti-ApoCIII (ab76305) with Alexa 488 and GFAP-568. This antibody is membrane bound present in astrocytes cells. Nucleus stained with Hoechst at high power magnification.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 Sep 06 2016