Application
Western blot
Sample
Human Cell lysate - whole cell (BREAST (MCF-7))
Loading amount
0.2 µg
Specification
BREAST (MCF-7)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: MILK
Secondary antibody
Name
Non-Abcam antibody was used: IgG
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 11 kDa
Additional data
Additional Notes
Monoclonal secondary antibodies were used in this experiment.The fuzzy images can be explained by either the low protein concentration 0.2ug,or poor image quality.
Abcam response
Thank you for your honest and valuable feedback. Have you considered increasing the protein loading amount (we suggest 20 ug) and using a polyclonal secondary antibody? We recommend retesting with these changes in order to confirm these results. Thank you for your time- we hope this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 01 2010