Application
Western blot
Sample
Mouse Tissue lysate - whole (liver, brain)
Loading amount
15 µg
Specification
liver, brain
Gel Running Conditions
Reduced Denaturing
Blocking step
5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: stabilized Goat anti-Rabbit HRP-conjugated(PIERCE)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL(West Dura)
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~220 kDa Non-specific: 170, 55 kDa
Additional data
Additional Notes
ApoB48 expressed in rodent liver. A specific band around 220 kDa is detected with ab31992 in mouse liver. It predicts this band comes from apoB48.
We didn't optimize the running condition for large protein in this blot. That is why we couldn't see a signal around 515KDa.
We didn't optimize the running condition for large protein in this blot. That is why we couldn't see a signal around 515KDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 11 2008