Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary astrocyte culture)
Permeabilization
Yes - 0.1M PBS with 1% Triton X
Specification
Primary astrocyte culture
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Methanol
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 0.1 M PBS with 1% TritonX
Dilution
1/500
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Alexa 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Rat primary Astrocyte culture stained with RAB MAB anti-ApoAII (Ab92478) with Alexa 488 and GFAP-568. This is a cholesterol transporter present in soma of astrocytes and transport to the growth cones. At higher magnification showed colocalisation with GFAP antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 Sep 06 2016