Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
10 µg
Specification
Liver
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20 %)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-goat IgG-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ~25 kDa Non-specific: ~45 kDa
Additional data
Additional Notes
The non-specific band around 45 kDa appears to be more prominent than the specific band at 25 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 02 2012