Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (4-20% tris-Glycin gel)
Sample
Human Cell lysate - whole cell (Platelet Activating Factor cancer cell lines)
Specification
Platelet Activating Factor cancer cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: #7074S anti-rabbit IgG Cell Signaling
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ez-ecl
Bands
Specific: 38, 40 kDa
Exposure
3 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 17 2014