Anti-Annexin-2/ANXA2抗体[M298] (ab86709)
Key features and details
- Mouse monoclonal [M298] to Annexin-2/ANXA2
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG1
概述
-
产品名称
Anti-Annexin-2/ANXA2抗体[M298]
参阅全部 Annexin-2/ANXA2 一抗 -
描述
小鼠单克隆抗体[M298] to Annexin-2/ANXA2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat -
免疫原
Recombinant fragment corresponding to Rat Annexin-2/ANXA2 (C terminal).
-
阳性对照
- WB: PC-12 cell lysate. ICC/IF: PC-12 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
M298 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86709于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 36 kDa.
|
|
ICC/IF |
1/50.
|
说明 |
---|
WB
1/1000. Detects a band of approximately 36 kDa. |
ICC/IF
1/50. |
靶标
-
功能
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
序列相似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
结构域
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻译后修饰
Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
-
数据库链接
- Entrez Gene: 56611 Rat
- SwissProt: Q07936 Rat
- Unigene: 90546 Rat
-
别名
- Annexin A2 antibody
- Annexin II antibody
- Annexin II, heavy chain antibody
see all
图片
-
Lane 1 : Anti-Annexin-2/ANXA2 antibody [M298] (ab86709) at 1/250 dilution
Lane 2 : Anti-Annexin-2/ANXA2 antibody [M298] (ab86709) at 1/1000 dilution
All lanes : Rat PC12 cell lysate
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Paraformaldehyde-fixed, NP-40 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) stained for Annexin-2/ANXA2 using ab86709 at 1/50 dilution in ICC/IF. Secondary antibody is a Goat antiMouse DyLight®594 conjugate.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab86709 尚未被引用在任何文献中。