Anti-Angiotensinogen抗体(ab97381)
Key features and details
- Rabbit polyclonal to Angiotensinogen
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Angiotensinogen抗体
参阅全部 Angiotensinogen 一抗 -
描述
兔多克隆抗体to Angiotensinogen -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Angiotensinogen aa 1-446.
Sequence:MRKRAPQSEMAPAGVSLRATILCLLAWAGLAAGDRVYIHPFHLVIHNEST CEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDK LRAAMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGA LDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQ AQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEK IDRFMQAVTGWKTGCSLMGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEF WVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVPFTESACLLLIQPHYA SDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAE LPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQL
Database link: P01019 -
阳性对照
- WB: HepG2 lysate. Mouse and Rat plasma. IHC-P: Hepatocellular carcinoma Huh7 xenograft. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97381于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/100 - 1/250.
|
说明 |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/250. |
靶标
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功能
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
Angiotensin-3 stimulates aldosterone release.
Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. -
组织特异性
Expressed by the liver and secreted in plasma. -
疾病相关
Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
序列相似性
Belongs to the serpin family. -
翻译后修饰
Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 183 Human
- Entrez Gene: 11606 Mouse
- Entrez Gene: 24179 Rat
- Omim: 106150 Human
- SwissProt: P01019 Human
- SwissProt: P11859 Mouse
- SwissProt: P01015 Rat
- Unigene: 19383 Human
see all -
别名
- Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
- AGT antibody
- AI265500 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma Huh7, using Angiotensinogen antibody (ab97381) at 1/100 dilution.
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Anti-Angiotensinogen antibody (ab97381) at 1/1000 dilution + Rat plasma at 50 µg
Predicted band size: 53 kDa -
HepG2 cells stained for Angiotensinogen using ab97381 at 1/200 dilution in ICC/IF.
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Anti-Angiotensinogen antibody (ab97381) at 1/1000 dilution + Mouse plasma at 50 µg
Predicted band size: 53 kDa -
Anti-Angiotensinogen antibody (ab97381) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 53 kDa
10% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab97381 尚未被引用在任何文献中。