重组
RabMAb

Anti-Angiotensinogen抗体[EPR2931] (ab108334)

概述

  • 产品名称
    Anti-Angiotensinogen抗体[EPR2931]
    参阅全部 Angiotensinogen 一抗
  • 描述
    兔单克隆抗体[EPR2931] to Angiotensinogen
  • 特异性
    ab108334 is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed.
  • 经测试应用
    适用于: WB, IP, ICC/IFmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • 阳性对照
    • Human fetal artery, fetal heart, fetal liver, adult kidney, HepG2, 293T, and plasma lysates; HepG2 cells.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

应用

Our Abpromise guarantee covers the use of ab108334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.

Block with 5% BSA

IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能
      Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
      Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
      Angiotensin-3 stimulates aldosterone release.
      Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
    • 组织特异性
      Expressed by the liver and secreted in plasma.
    • 疾病相关
      Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
      Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
    • 序列相似性
      Belongs to the serpin family.
    • 翻译后修饰
      Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
      • AGT antibody
      • AI265500 antibody
      • Alpha 1 antiproteinase antitrypsin antibody
      • Ang antibody
      • Ang I antibody
      • Ang II antibody
      • Ang III antibody
      • AngII antibody
      • Angiotensin I antibody
      • Angiotensin II antibody
      • Angiotensin III antibody
      • Angiotensin-3 antibody
      • Angiotensinogen (PAT) antibody
      • Angiotensinogen antibody
      • ANGT_HUMAN antibody
      • ANHU antibody
      • ANRT antibody
      • AT-2 antibody
      • AT-II antibody
      • Des-Asp[1]-angiotensin II antibody
      • FLJ92595 antibody
      • FLJ97926 antibody
      • MGC105326 antibody
      • PAT antibody
      • Pre angiotensinogen antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serpin A8 antibody
      • Serpin peptidase inhibitor clade A member 8 antibody
      • SERPINA8 antibody
      see all

    图片

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Mouse kidney whole cell lysate
      Lane 2 : Rat kidney whole cell lysate
      Lane 3 : Human kidney whole cell lysate
      Lane 4 : Mouse liver whole cell lysate
      Lane 5 : Rat liver whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

      Predicted band size : 53 kDa


      Exposure time : 1 minute

      Ab108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).

      Blocking buffer for the first lanes 1-5: 5% NFDM/TBST; the second lanes 1-5: BSA/TBST.

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Human fetal liver whole cell lysate
      Lane 2 : Human fetal liver whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

      Predicted band size : 53 kDa


      Exposure time : 3 minutes

      Lane 1 blocking buffer: NFDM/TBST

      Lane 2 blocking buffer: BSA/TBST

    • All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

      Lane 1 : Human fetal artery lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : HepG2 lysate
      Lane 5 : 293T lysate
      Lane 6 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 53 kDa
    • ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence.

    文献

    This product has been referenced in:
    • Xiang X  et al. Tuberous sclerosis complex 1-mechanistic target of rapamycin complex 1 signaling determines brown-to-white adipocyte phenotypic switch. Diabetes 64:519-28 (2015). Read more (PubMed: 25213336) »
    • Kemp JR  et al. Angiotensin II-regulated microRNA 483-3p directly targets multiple components of the renin-angiotensin system. J Mol Cell Cardiol 75:25-39 (2014). Read more (PubMed: 24976017) »

    See all 7 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (10% PAGE)
    Loading amount
    25 µg
    Specification
    Cardiomyocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 28 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (human embryonic stem cells)
    Specification
    human embryonic stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    提交于 Jun 06 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (epididymis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    epididymis
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    bouin's solution
    Username

    Abcam user community

    Verified customer

    提交于 Apr 09 2013

    Thank you for contacting us. Antibodies are OK at room temperature for about 1 a week. We use this time to ship antibodies to our customers although we include icepacks in order to keep them at 4C in case of any unnecessary delays. Once receive...

    Read More

    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments.   There is a very clear, single band on the customer's blot at 34 kDa, though the predicted band of precursor 53 kDa.   During ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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