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Thank you for reply. I have used previously ab3510 successfully with a lot. 515591, and also perhaps with another previous lot that I have not stored. Those other lots mentioned below have not worked at all. Please enclosed find the Abcam WB Questionnaire for info of protocols used. Now I have used Sigma that works fine. Please enclosed find the results of my test runs with Abcam vs. Sigma antibodies (identical runs except the different primary antibody). I do not know why Product manager xxxx did not send this file already to you.
To me there are no doubts that your antibody did not work. Typically, we observe multiple bands with AR, and especially a strong un-specific band at ˜60-70 kDa with the skeletal muscle samples. That is not a correct AR band. I except that you somehow compensate to me the time and money that I have been lost with your product. Furthermore, I have to admit that I am very upset of the slow process of my reclamation, I have send this to xxxxxx already at April 11th 2011.
Asked on Mar 13 2012
Thank you for taking time to compile that data and sharing it with me.It is quite clear that this antibodyhas not been performing satisfactorily in your experiments. The details provided will enable us to investigate this case further and will provide us with vital information for monitoring product quality. I thank you for bringing this problem to our attention.
I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. I am not sure why we were not forwarded this information by xxx but that is why I decided to contact you directly. We take customer complaints very seriously and aim to provide a response within 24 hours of receiving any correspondence. If you have a similar situation in the future where you have not heard from us (through xxx), please do contact us directly. As you are in Finland, you can also purchase directly from us if you prefer.
Having reviewed the data and the protocol details, I believe this product should have given satisfactory results, but it clearly has not.This is a littleperplexing as the original lot which you found to work (515591) was from the same bleed as the subsequent lots. It appears that something may have happened in the handling of the different batches. We will be looking into this further. I have requested that the antibody be retested. If you could share with me the dataobtained with lot515591 that would be veryhelpful in aiding our internal investigation further.
I apologize for the inconvenience this problem has caused you. Although the Abpromise technically only covers a product for 6 months following purchase, in this case I am willing to extend the cover and offeryou a replacement or a refund of all the lots you have found not to be functional. In order to do this I will need to know the order numbers of each purchase. I have looked back at our records and have found two orders that I think are yours:
Order number 659726 (18/03/2010) and 659080 (17/03/2010) both delivered to xxxxx(xxxxx Finland) through xxxxx.
I cannot find reference to any other orders. Did you use a different delivery address prior to 2010? I can probablyfind the additionalorders ifyou can giveme a delivery address and an approximatedate of the order.
We have several alternative antibodies to Androgen Receptor but the one that I would suggest trying as an alternative is http://www.abcam.com/Androgen-Receptor-antibody-EP670Y-ab52615.html. This is a rabbit monoclonalwhichhave been found to havevery goodbindingproperties. This antibody hasbeen successfully used in westernblotting of the protein (clean band at the expectedsize)and is compatible withmouse, human and rat samples. If you would like, I can arrange for you tofirst try alot of this antibody, thenif you don'tlike the results, you can decide to have the full refund ofall your faulty products.
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.
Abcam Scientific Support
回复于 Mar 13 2012