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You have a very nice picture of a mouse hypothalamus immunostained with this antibody, could you tell me what dilution of antibody was used to acheive this? The notes on immunohistochemistry just say use an assay-dependent dilution, but if you've done it already....
Asked on Mar 01 2006
I was informed by the source of ab3510 that the reason why there is no recommended dilution for ab3510 is that the current lot has not been tested in IHC and therefore we do not know what dilution it should be used at. In light of this we are currently considering removing IHC as a tested application and removing the image too. I would recommend to try a range of dilutions in a preliminary experiment (1:10, 1;50, 1:100, 1:200, 1:500) and optimizing following the results. I'm sorry I couldn't help you more. If you experience problems please do not hesitate to contact me and I can look into details of your protocol to help more and should the problem be antibody related offer you a refund,
Abcam Scientific Support
回复于 Mar 02 2006