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Synthetic peptide corresponding to Human Androgen Receptor (N terminal). Sequence is propreitary.
Our Abpromise guarantee covers the use of ab74272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. PubMed: 24466341|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21430025|
ab74272 used in ChIP application.Whole cell lysate from human LNCaP cells was used.Cross linking step (X-ChIP) for 10 minutes in 1% formaldehyde.Taqman detection step.Positive control was PSA enhancer.Negative control region, no antibody control, and in the absence of ligand.25 µl undiluted ab74272 per IP was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma labelling Androgen Receptor with ab74272 at a dilution of 1/200 for 30mins at room temperature.
ab74272 staining Androgen Receptor in mouse neuroblastoma cells by Immunocytochemistry/Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour. The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C. The secondary antibody was Alexa Fluor® 594-conjugated goat anti-rabbit polyclonal, diluted 1/400.
ab74272 staining Androgen Receptor in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA and blocked with 5% BSA for 45 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 1% BSA) for 16 hours at 4°C. A TRITC-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
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