Application
IHC - Wholemount
Sample
Drosophila melanogaster Tissue (Isolated central nervous system)
Specification
Isolated central nervous system
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% Triton X-100 + 2% BSA
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
UAS-CD8-GFP (membrane bound GFP) was expressed in the Giant Fiber neurons using the R68A06-Gal4 driver in a wild-type genetic background. Image taken of the Giant Fiber terminals located within the thoracic ganglion of the Drosophila central nervous system. Sample was co-incubated with anti-GFP and anti-APP[Y188]. Image clearly shows that APP[Y188] (ab32136) does not detect endogenous Drosophila APP (APPL) in an IHC preparation performed under the previously described conditions. Bright red puntca appear to be non-specific.
Abcam response
Thank you for testing ab32136 in Drosophila melanogaster, an untested species. We welcome any other feedback in this species by participation in our AbTrial program: www.abcam.com/abtrial
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 02 2017