The ammonia determination is accurate for samples of tissue extracted in methanol:acetonitrile:water (5:3:2) solution, provided that the samples are diluted 1:5 in assay buffer. The reaction efficiency decreases when the ratio between organic solvents and assay buffer increases. The reaction does not occur if the samples in organic solvents are not further diluted in assay buffer.
Figure Legend. The standard curve has been generated by mixing in each well 10 microliters of methanol, acetonitrile, water, solution containing known amounts of ammonia, to 40 microliters of assay buffer. The reaction started with the addition of 50microliters/well of reaction mix. Samples were incubated at 37 degrees for 1 hour.
Figure Legend. The standard curve has been generated by mixing in each well 10 microliters of methanol, acetonitrile, water, solution containing known amounts of ammonia, to 40 microliters of assay buffer. The reaction started with the addition of 50microliters/well of reaction mix. Samples were incubated at 37 degrees for 1 hour.
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提交于 Jul 27 2016