Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Specification
SH-SY5Y
Treatment
50 ´M, 100 ´M or 200 ´M of dopamine with or without 100 nM of MG132 proteasome inhibitor for 72 h
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% milk inn PBS with 0.05% v/v Tween-20
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL Femto
Bands
Specific: 30, 50, 160 kDa
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
At lower concentrations of dopamine, lower-ordered oligomeric species such as dimers and trimers form and upon increasing the dopamine concentration, there is a shift to higher ordered oligomeric species. Using the dopamine in combination with MG132 proteasome inhibitor prevents the degradation of oligomeric species and therefore they are able to accumulate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. B Roberts
Verified customer
提交于 Feb 09 2015