Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (cerebral cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6.0, 20 min, 100C
Permeabilization
No
Specification
cerebral cortex
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Leice Refine detection,anti-mouse anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
IHC procedure fully automated on Leica Bond RX
Various retrieval regimens tested (none, proteinase, citrate, EDTA). Citrate gave best signal to background reaction.
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提交于 Dec 16 2016