重组Anti-NEAS抗体[EPR3017] (ab75755)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3017] to NEAS
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-NEAS抗体[EPR3017]
参阅全部 NEAS 一抗 -
描述
兔单克隆抗体[EPR3017] to NEAS -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human NEAS aa 2450-2550. The exact sequence is proprietary.
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阳性对照
- WB: HeLa lysates, untreated and treated with Camptothecin; Jurkat whole cell lysate (ab7899), untreated and treated with Staurosporine; TF-1, SH-SY5Y and human brain lysate, treated and untreated HeLA and parental HAP1 whole cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3017 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75755于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/500 - 1/2000. Detects a band of approximately 285 kDa (predicted molecular weight: 285 kDa).
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说明 |
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WB
1/500 - 1/2000. Detects a band of approximately 285 kDa (predicted molecular weight: 285 kDa). |
靶标
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功能
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
疾病相关
Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia. -
序列相似性
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats. -
翻译后修饰
Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. - Information by UniProt
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数据库链接
- Entrez Gene: 6709 Human
- Entrez Gene: 64159 Rat
- Omim: 182810 Human
- SwissProt: Q13813 Human
- SwissProt: P16546 Mouse
- SwissProt: P16086 Rat
- Unigene: 372331 Human
- Unigene: 204969 Mouse
see all -
别名
- (ALPHA)II-SPECTRIN antibody
- Alpha II Spectrin antibody
- Alpha-II spectrin antibody
see all
图片
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All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa, treated with 1 µM Staurosporine for 3 hours, whole cell lysate
Lane 3 : Untreated parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
Lane 4 : parental HAP1, treated with 1 µM Staurosporine for 3 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 285 kDaMerged signal (red and green). Green -ab75755 observed at 285,150,120 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) as secondary antibody.
Red - loading control, ab7291 observed at 52 kDa using Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) as secondary antibody.
Ab75755 was shown to react with NEAS in HeLa and parental HAP1 cells. lower signal was observed when treated cells (1 µM Staurosporine for 3h) was used. Ab75755 and ab7291 (Mouse anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with ab216773 and ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution
Lane 1 : HeLa cell lysate treated with Camptothecin
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat treated with Staurosporine
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 285 kDa
Additional bands at: 120 kDa (possible cleavage fragment), 150 kDa (possible cleavage fragment), 85 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/2000 dilution
Lane 1 : TF-1 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 285 kDa
Additional bands at: 150 kDa (possible cleavage fragment)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab75755 被引用在 9 文献中.
- Tsuchiya K et al. Gasdermin D mediates the maturation and release of IL-1α downstream of inflammasomes. Cell Rep 34:108887 (2021). PubMed: 33761363
- Chen YH et al. Deficiency in Androgen Receptor Aggravates Traumatic Brain Injury-Induced Pathophysiology and Motor Deficits in Mice. Molecules 26:N/A (2021). PubMed: 34684832
- Lam PM & González MI Calpain activation and neuronal death during early epileptogenesis. Neurobiol Dis 124:141-151 (2019). PubMed: 30423475
- Zhang T et al. Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload. Neuroscience 411:86-104 (2019). PubMed: 31129202
- González MI Calpain-dependent cleavage of GABAergic proteins during epileptogenesis. Epilepsy Res 157:106206 (2019). PubMed: 31585309
- Lam PM et al. A calpain inhibitor ameliorates seizure burden in an experimental model of temporal lobe epilepsy. Neurobiol Dis 102:1-10 (2017). PubMed: 28237317
- Yan M et al. Effect of glutamate on lysosomal membrane permeabilization in primary cultured cortical neurons. Mol Med Rep 13:2499-505 (2016). PubMed: 26821268
- Campos-Martorell M et al. Brain proteomics identifies potential simvastatin targets in acute phase of stroke in a rat embolic model. J Neurochem 130:301-12 (2014). PubMed: 24661059
- Sebe JY et al. ALLN rescues an in vitro excitatory synaptic transmission deficit in Lis1 mutant mice. J Neurophysiol 109:429-36 (2013). WB . PubMed: 23100132