Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult kidney)
Specification
adult kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300
Additional data
Additional Notes
Kidney localisation in rat appears to confirm findings in mouse and human kidney, under same conditions. However, I am very surprised at the extent of expression in rat. Images clearly show intense widespread expression in the collecting tubules of the hilar region. In the cortical region expression appears to be quite localised. In the transitional epithelium of the ureter, in the Hilar region of the kidney, there is intense, cytoplasmic immunostaining with this antibody ( image not shown. Please see here for additional images : http://www.immunoportal.com/)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Oct 21 2008