Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19)
Gel Running Conditions
Reduced Denaturing (4-12% bolt gel)
Loading amount
15 µg
Treatment
125 ug/ml cigarette smoke extract, 12 hr
Specification
ARPE-19
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: HRP-linked whole Ab from donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
none
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: none kDa
Positive control
cse stressed ARPE-19 cells
Additional data
Additional Notes
1 – ARPE19 cells w/ DMSO 12 h
2 – ARPE19 cells w/ Cigarette Smoke Extract 12 h
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 26 2017