Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
30 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/300
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% Milk/TBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 70 kDa
Additional data
Additional Notes
The additional 70kDa band may represent a post-translationally modified form (e.g. phosphorylated) of alpha 2-antiplasmin, but this was not confirmed
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Rachel Barker
Verified customer
提交于 Nov 12 2010