Aldehyde Assay试剂盒(Colorimetric) (ab112113)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Plasma, Saliva, Suspension cells
概述
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产品名称
Aldehyde Assay试剂盒(Colorimetric)
参阅全部 Aldehyde 试剂盒 -
检测方法
Colorimetric -
样品类型
Saliva, Plasma, Cell culture extracts, Adherent cells, Suspension cells -
检测类型
Quantitative -
种属反应性
与反应: Mammals, Other species -
产品概述
Aldehyde Assay Kit (Colorimetric) ab112113 is a a sensitive, one-step colorimetric assay to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step.
The aldehyde assay protocol uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS.
The signal is read with an absorbance microplate reader at 405 or 550 nm.
Aldehyde assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30-60 min
- analyze with a microplate reader -
说明
Previously called Aldehyde Quantification Assay Kit (Colorimetric).
This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.
ab112113 should be stored desiccated.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 200 tests Aldehyde Standard 1 vial Assay Buffer 1 x 10ml Dilution Buffer 1 x 20ml Yellow Indicator 2 units -
研究领域
图片
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Aldehyde measured in biological fluids showing quantity (uM) per mL of tested sample. Values calculated based on the 550 nm Standard Curve. Samples diluted 1-10 fold.
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Aldehyde measured in cell culture supernatants showing quantity (uM) per mL of tested sample. Values calculated based on the 405 nm Standard Curve. Samples diluted 2 fold.
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Aldehyde measured in cell lysates showing quantity (uM) per 1 mln cells. Values calculated based on the 550 nm Standard Curve.
Samples with the concentration of 1.26e8 cells/mL (HeLa) and 1e8 cells/mL (HepG2) were used. Samples were diluted 3 fold.
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Aldehyde dose response was measured in a 96-well black plate with ab112113. As low as 10 µM (1 nanomol/well) of aldehyde can be detected with 30 minutes incubation (n=3). Standard curve was read at 405 nm.
数据表及文件
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Datasheet download
文献 (3)
ab112113 被引用在 3 文献中.
- Hsueh CY et al. A positive feed-forward loop between Fusobacteriumnucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis. iScience 25:103829 (2022). PubMed: 35198889
- Boby N et al. Ethanol-Induced Hepatotoxicity and Alcohol Metabolism Regulation by GABA-Enriched Fermented Smilax china Root Extract in Rats. Foods 10:N/A (2021). PubMed: 34681429
- Li C et al. Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome. Nat Commun 8:1257 (2017). PubMed: 29097652