Application
Western blot
Sample
Rat Tissue lysate - whole (white adipose tissue)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Specification
white adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
NA
Exposure
30 second(s)
Bands
Specific: 50 kDa Non-specific: 64, 35 kDa
Positive control
NA
Additional data
PubMed ID
25703837: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. William Zhang
Verified customer
提交于 Mar 06 2018