Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Articular Cartilage)
Specification
Articular Cartilage
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic
Blocking step
Protein Block Serum-Free (casein in PBS) as blocking agent for 5 minute(s) · Concentration: 0.25
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Envision+HRP Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
We tried multiple retreival methods and found that pronase and hyaluronidase will work. The best results were with hyaluronidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Elizabeth Chlipala
Verified customer
提交于 Mar 21 2007