Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF, R1 ES cells, E14 ES cells)
Specification
MEF, R1 ES cells, E14 ES cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor donkey anti mouse A1003
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000
Additional data
Additional Notes
A colony of E14 mES cells on top of MEF cells, following 16 hours of VPA treatment. Aggrecan antibody stains cytoplasm, in the mES colony appears as round empty circles. An additional dapi picture can be sent, 2 pictures cannot be uploaded.
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提交于 Jun 20 2011