Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Spermophilus tridecemlineatus Tissue lysate - whole (white adipose tissue)
Specification
white adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% BSA in TBS-T
Dilution
1/2500
Secondary antibody
Name
Non-Abcam antibody was used: Immunopure
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
Supersignal West Pico
Bands
Specific: 29 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
white adipose tissue - adiponectin is known to be produced by this tissue.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 31 2014